Ongoing Research Projects

A.        Externally funded (Foreign) : Nil

B.        Externally funded (National)

Title

Investigators

Brief Objectives

Duration

Brief highlights

Total outlay (Rs. in lakhs)

APEDA National Referral Laboratory for Monitoring Pesticide Residues for Export of Fresh Grapes from India

Director (S.D. Sawant), PI

Kaushik Banerjee

  • Monitoring of the pesticide residues in exportable fresh table grapes to the European Union through control of residues of chemicals

01.04.2012 to 31.03.2017

Develop and validate analytical methods for analysis of pesticide residues in Grapes

Monitoring of exportable and domestic production for residue free production of table grapes

3586.22

Validation of descriptors for Distinctiveness, Uniformity and Stability for grape varieties under Indian conditions J. Satisha

Roshni R. Samarth

  • Development of valid DUS Test Guidelines for Grapes under Mild Tropical Conditions
  • To establish Salient features/identifiers of relevant grape cultivars.
  • To create national documentation of the cultivars in use for their registration at national level.
  • To protect the rights of plant breeders and farmers responsible for new variety development.
  • Validation of data of descriptors for Grapes under subtropical and temperate Indian conditions.
  • The protocol will also help in introduction and protection of exotic / patented cultivars under Indian conditions under sui-generis system.
01.02.2012 to 31.07.2015 25.00

Functional analysis of salinity stress response in grapevine

Anuradha Upadhyay, PI
 A.K. Upadhyay
 J. Satisha

  • To understand the molecular response of grapevine to salinity stress at transcript and proteome levels
  • To understand the physiological mechanism of salt tolerance in grapes and interaction among different cellular responses to salinity stress
  • To study the mechanism of stress tolerance in rootstock and its influence on gene expression in scion variety under stress
  • To identify promising salinity tolerance genes from grapes
    To develop functional markers for salinity tolerance in grapes

20.11.2012 to 20.11.2015

RNA sequencing based global analysis of transcriptome as well proteome analysis will be used to understand the molecular and metabolic response of grapevine to salinity stress. Comparison of the RNAseq and proteomics data with the available draft genome sequence of grape will allow identification of structural and regulatory genes, novel transcripts including splice variants, non-coding RNAs, key proteins, changes in protein expression, post-translational modifications in proteins and genomic regions controlling stress tolerance, all of which could be used for grape improvement.

69.84

 

C.  Institute / ICAR

Title

PI with associates

Brief Objectives

Duration

Brief highlights

Total outlay (Rs. in lakhs)

Management of  Grape Genetic Resources  of  table, wine, raisin, juice and rootstock varieties - Phase II

R.G. Somkuwar, PI
Anuradha Upadhyay

A.K.Sharma

A.K.Upadhyay

Indu S. Sawant

S.D. Ramteke

D.S. Yadav

·       To evaluate cultivars as active collections

·       To conserve desirable genotypes on long term basis through cryo-preservation in collaboration with NBPGR, New Delhi.

·       To exchange and coordinate the grape improvement programme as a nodal centre in the country.

01.04.2008 to 30.06.2015 (Phase II)

This project forms a basis for establishing a sound national germplasm repository for grapes at NRCG, Pune. Collection of clonal material will be expedited through surveys and explorations and also augmentation from the previous collections from different AICRP (STF) grape centers in the country. Thrust will be given for tolerant types to biotic and abiotic stresses. The maintenance of clonal material will be in the form of active field   gene bank on midterm basis (12-15 years). However, long term  germplasm  conservation  efforts will be taken up, when suitable facilities and expertise  for tissue culture /cryopreservation will be established in collaboration with NBPGR, New Delhi.

The technical programme will be directed towards the following areas:

1.   establish gene bank of various traits of economic importance,

2.   identify suitable donors for biotic and abiotic stress tolerance,

3.   establish relevant molecular markers for cultivar identity and diagnostics,

4.   evolve improved cultivars from the base (check) Thompson seedless or introduce superior genotypes widely acceptable for commercial cultivation.

 All the accessions will be maintained  as grafted plants on a standard rootstock (DogRidge).These  will be identified in separate groups based on their end use such as table grapes, raisin grapes, wine grapes, juice grapes and rootstocks. Closely related  species/genera  will also be collected and studied for use in grape improvement through hybridization and crop improvement.

158.80

Development of new grape varieties through conventional breeding

 

Roshni Samarth, PI

Anuradha Upadhyay

 

·       To study the inheritance of economically important traits through hybridization

·       To develop viable alternate   varieties  suitable for table, raisin, juice and   wine   making.

·       To  identify clonal selections of  both natural and  induced  mutants with  improved  plant &  fruit characters by  molecular markers .

·       To develop tolerant grape varieties and rootstocks to biotic and  abiotic stress

·       To develop marker assisted selections  for  economically  important   traits.

·       To develop  right  protected varieties and rootstocks.

01.10.2008 to 31.05.2013

Several grape varieties are known to possess resistant genes against downy mildew. A cross between two seedless varieties often results into embryo abortion resulting in no progeny plants. It was  possible to rescue hybrid embryos as soon as they are formed but before their abortion and raise hybrid progeny. Several hybrid plants have been obtained having considerable resistance to diseases but mostly turned out be seeded. Further crossing with preferred seedless parents is necessary to bring about satisfactory introgression of the traits. Similarly improved rootstocks are required through breeding  for tolerance to  low /high soil moisture and high soil salinity. Thus it is possible to develop new grape varieties by  conventional breeding method and use of DNA molecular markers to ascertain hybrid progenies at seedling stage itself is the most recent and preferred approach worldwide. 

108.95

In Silico identification of abiotic stress (salinity) responsive transcription factors and their cis-regulatory elements in grape

Dr.Anuradha Upadhyay, PI

Dr.A.K.Upadhyay,

Sarika, Scientist (Agricultural Statistics)

IASRI, New Delhi.

·       To identify stress responsive structural genes and Transcription Factors in grape.

·       To identify cis acting elements in the promoter regions of TFs.

·       Comparative genomics analysis of selected transcription factors.

·       Validation of expression of selected genes under stress conditions by real time PCR

01.01.2012 to 31.12.2013

 In India, grape cultivation is concentrated in the semi arid region. Unpredictable rains, poor irrigation water quality, excess use of fertilizers, intensive cultivation, poor farm management and poor drainage adds to the already prevalent saline soil conditions. Productivity is declining for past 10-15 years in major grape growing regions of the country due to problems associated with drought and soil and water salinity. The understanding of salinity stress will be greatly enhanced by elucidating the structural and regulatory genes responsible for developmental and physiological processes under stress response. Besides several structural genes, Transcription Factors (TFs) are the key molecular switches controlling key biological processes. The 12X genome sequence of the grape with the V0 version of the gene annotation is available in public domain. The sequence information can be used for searching the candidate genes using comparative genomics and prediction model approaches.

 41.02

Breeding for Development of Marker Assisted Selection (MAS) Technique for Downy Mildew Resistance in  Seedless grape varieties.

Roshni R. Samarth, PI

Anuradha Upadhyay

Indu S. Sawant

 

·       To carry out crossing programme for transferring downy mildew resistant trait in Thompson seedless.

·       To confirm the hybrid nature of the seedlings using microsatellite markers.

·       To study the co-segregation of markers and disease resistance trait in F1 population.

·       To develop technique for marker assisted selection.

·       To develop a downy mildew resistant variety of Thompson seedless

01.09.2010 to 31.08.2015

The primary aim of the project is, breeding to develop a mapping population segregating for the downy mildew resistant trait in Thompson seedless variety of the grape using Sevye villard 23501 as a donor parent. The project will be initiated by carrying out the crossing programme and the confirmation for transfer of the character will be done by molecular markers in F1 generation. This will also be facilitated by in-vitro screening for the character.

95.81

Clonal selection in grapes

Roshni R. Samarth,PI
R.G. Somkuwar
Anuradha Upadhyay S.D. Sawant

·       To identify the clones/mutants of popular grape varieties with improved traits.

·       Confirmation of the identified mutants using microsatellite markers.

01.04.2011 to 29.02.2016

The survey will be carried out in the grape vineyard in different parts of the Maharashtra to identify the vine with desirable quality. First, morphological observations will be recorded in the farmer’s field for three years continuously and promising collection will be identified. Serological test through ELISA will be carried out to test the presence of virus in the identified vines. Simultaneously the molecular analysis will also be carried out to confirm the mutation. Healthy vines will be carried forward for multiplication at the research field and observations will be recorded and will be compared with the vines at farmer’s field.    

127.14

Proteomic analysis of Thompson Seedless grapes grafted on different rootstocks at different phenological stages of growth and development

J. Satisha, PI

Anuradha Upadhyay

Kaushik Banerjee 

·       To characterize the proteomic profile in Thompson Seedless grapes at different stages of vine growth and development.

·       To study the effect of rootstocks in influencing the protein profile in Thompson Seedless at different phenological stages.

·       To understand the overall mechanism of rootstocks in controlling vegetative, reproductive and quality attributes of scion varieties with respect to their role played in regulating different proteins / enzymes.

29.04.2011 to 26.12.2014

Of the four protocols compared for protein extraction, resolution of protein in 1st dimension and 2nd dimension gel was improved with modified trichloro acetic acid/acetone precipitation coupled with phenol extraction method. This method also yielded higher protein yield, greater spot resolution with minimal streaking on 2D gels. The same protocol could yield higher quality protein from other grape tissues such as buds, berries and roots. This protocol can give good quality protein with satisfactory and reproducible separation on 2D gels.

Among different stocks scion combinations, Thompson Seedless grafted on 110 R rootstocks and own rooted vines imparted maximum bud burst percentage after forward pruning which was attributed to increased peroxidase and polyphenol oxidase activity in their buds and also to highest protein content. Maximum bud fruitfulness was recorded on 110R rootstock and own rooted vine.

Analysis of hormones at different stages of berry development revealed definite trend. Endogenous IAA was highest during initial stages of berry development (1st two stages); while GA3 concentration was highest till 8-10 mm berry size (responsible for cell enlargement and ABA concentration was highest at Veraison stage and later on there was decrease in its concentration.

66.51

Evaluation of rootstocks for growth ,yield, fruit composition and wine quality of Cabernet Sauvugnon grapes grown in Pune region of India.

J. Satisha

R.G. Somkuwar

J. Sharma
A.K. Upadhyay A.K. Sharma

·       To study the influence of rootstocks on growth and development of Cabernet Sauvignon grapes.

·       To study the influence of rootstocks on fruit composition, must and wine quality of Cabernet Sauvignon grapes.

·       To study the influence of rootstocks on mineral nutrient uptake / accumulation in Cabernet Sauvignon wine grapes.

·       To study the influence of rootstocks on incidence of diseases in Cabernet Sauvignon grapes.

01.01.2012 to 30.06.2017

Cabernet Sauvignon grape vines grafted on seven different rootstocks were evaluated for vegetative, yield and fruit composition parameters. First year results indicated that, among different stock scion combinations, vines grafted on 140RU recorded maximum pruning weight while vines grafted on 101-14 Mgt recorded early bud sprouting while it was late on SO-4 rootstock. Maximum number of clusters was recorded on Gravesac while highest yield per vine was recorded on 101-14 Mgt which may be due to increased berry weight. Among fruit composition parameters significant difference was recorded for TSS, Juice pH and potassium content among rootstocks. Least potassium content was recorded on 110R rootstock which also recorded highest juice pH.

Petiole nutrient analysis at the time of veraison showed significant difference for phosphorus content where in 1103 P recorded highest P content while highest potassium content was recorded on 110R rootstock. Significantly least Sodium content of 0.18% was recorded on 110R and Fercal rootstocks while it was highest on SO-4 and 10.14 Mgt rootstocks.

90.905

Standardization of cultural practices to increase quality yield of wine grapes

R.G. Somkuwar, PI
J. Satisha
A.K. Sharma

J. Sharma

S.D. Sawant 

·       To standardize the training system.

·       To standardize vine spacing

·       To standardize pruning practices

·       To standardize bud load per vine

01.04.2010 to 31.03.2015

The experiments on standardization of pruning dates, planting density, crop load and different training systems in relation to quality wine were laid pout under the project. The vineyard is under infrastructure development stage.

42.60

Standardization of propagation techniques in grapes

R.G. Somkuwar, PI
S.D. Ramteke

J. Sharma

 

·       Effect of plant sap extract on rooting of promising grape rootstock and commercial varieties.

·       Effect of microbial inoculants on success of grape rootstock and commercial varieties multiplication through hardwood cuttings.

·       Economics of different methods of propagation in grapes

·       Effect of stock age of promising rootstocks on grafting success

·       Effect of scion-preconditioning on grafting success in different rootstocks.

01.04.2008 to 31.03.2013

Effect of different concentration of IBA on rooting of bud sticks of rootstocks

Different rootstocks responded differently for days taken for bud sprouts and per cent success of rooting. Minimum days taken for bud sprouts were reported in Freedom rootstocks and 1103 rootstock at 1000ppm concentration as compared to other rootstocks. Maximum rooting success and shoot length was recorded in the same rootstocks. The rootstocks 110R and B2/56 sprouted early with application of 1500 ppm IBA.

The study was also conducted on four different wine varieties Sauvignon Blanc, Cabernet Sauvignon, Chenin Blanc and Shiraz).  Among the different concentrations, minimum days for bud sprouts were noted in 1000 ppm of IBA with better rooting.

Effect of leaf removal on grafting success in Thompson Seedless grapes

The leaves from mother vine were removed at 8,6,4,2 days before grafting and were compared with the no leaf removal treatment. Bud sprouting was the earliest in leaf removal at 8- days followed by 6 days and 4- day treatment. The leaf removal before 8- days of grafting resulted in to 98% grafting success as compared to other treatments of leaf removal.

Effect of zinc (Zn) on rooting success through hardwood cuttings

Zinc at different concentration of 5, 10, 15 and 20g/mother vine was applied two month before harvest. Zinc application @5g/vine resulted for days taken for bud sprouts as compared to other treatments. The nutrient content in shoots of rooted cuttings recorded at 120th days after planting indicated the use of Zn @5g/plant for better success in rooted cuttings of Dog Ridge rootstock.

Root distribution studies of different grape rootstocks

The cuttings of different rootstocks (Dog Ridge, Salt Creek, 140-Ru and 110-R) were selected for study. Dog Ridge rootstock produced higher primary and secondary root length followed by Salt Creek. However, the lowest primary root length was recorded in 140-Ru rootstock. The highest root diameter was recorded in Dog Ridge rootstock whereas the lowest in 110-R rootstock. The rootstock Dog Ridge rootstock exhibited higher root biomass followed by Salt Creek, 140-Ru and 110-R rootstock.

23.42

Performance of wine varieties under Indian condition for yield and quality wine.

R.G. Somkuwar, PI
J. Satisha
A.K. Sharma

·       To evaluate the varieties under Indian conditions for important traits.

01.04.2011 to 31.03.2016

 The performance for growth, yield and wine quality was studied for nine white wine varieties and ten red wine varieties under Indian condition.

Significant differences were recorded for growth, yield and wine quality. Among the white wine varieties, the variety Gewurztraminer was early to sprout (8.84 days) compared to Muscat White (11.68 days). Higher shoot length of 70.24 cm was also recorded in Muscat White. Higher cane diameter of 7.18mm was recorded in Colambard variety with 93.35% fruitfulness. Higher number of bunches were recorded in Viognier (76.17) whereas the lowest number were recorded in Gros Menseng (3.0). Higher yield per vine was recorded in Chenin White (4.0 kg) than the lowest in Gros Menseng (0.68 kg). Significant differences were also recorded for wine quality.

Among the red wine varieties, the days for bud sprout ranged from 8.70 in Syrah to 13.90 in Petit Verdot. Higher shoot length of 74.52 cm was recorded in Niellucio compared to the lowest in Merlot (44.36). Cane diameter ranged from 6.30mm in Petit Verdot to 7.89mm in Grenache. The fruitfulness ranged from 57.30% in Cinsaut to 92.96% in Grenache. The average bunches per vine ranged from 17.56 in Cinsaut to 51.14 in Grenache. The yield/vine ranged from 0.76 kg in Syrah to 4.07kg in Grenache. Significant differences were also recorded for wine quality among the red wine group.

49.70

To evaluate the performance of Cabernet Sauvignon grapes raised on different rootstocks under various salinity levels

A.K. Upadhyay,PI

J. Sharma

S.D. Ramteke

R.G. Somkuwar

 

·       To evaluate the scion-stock combination for their salinity tolerance under pot conditions.

·       To study the nutrient distribution in plants under varying salinity levels and scion-stock combination.

·       ·        To monitor the physiological changes in the grapevine at various growth stage

01.04.2012 to 30.06.2015

Salinity has become a key production related constraint in worldwide agriculture. Managing salinity scientifically and effectively is the challenge before the Agricultural Scientists in the world. The problem of salinity is quite common in existing and new areas for grape cultivation in the state of Maharashtra and therefore, this problem has to be taken up seriously in view of economic production with quality fruits in grape growing areas in time to come in the future.

89.69

Standardizing irrigation schedule for Cabernet Sauvignon vines raised on 110R rootstock

A.K. Upadhyay, PI

J. Sharma,

A.K. Sharma

·       ·        To determine the appropriate sequence of water replenishment through various stages of vine growth and berry development

·       ·        To study the effect of irrigation treatments on yield and quality of grapes.

01.04.2010 to 30.06.2014

The technologies for quality wine grape production are still in the nascent stage in India. In fact systematic research is lacking in production technologies for wine grape cultivation. Cabernet Sauvignon is one of the wine varieties adapted to hot climate conditions. However, the cultivation practices being followed elsewhere are applied to our situation. This is not the right practice. Judicial application of water will not only help in maximizing the scarce water resource but at the same time also help in minimizing the salinity risk. Most of the vineyards in India are drip irrigated. The advantage of this irrigation technique lies in the precise control over time, quantity and desired area of water application. Amongst the various inputs applied to the crop, water is the most important input.

38.25

Decision Support System for Enhancing Productivity of Grapes under Moisture and Temperature Stress Conditions

J.Sharma, PI
A.K.Upadhyay  J. Satisha

  • To study the effect of moisture and temperature stress on growth and yield of grapes to generate data library required for crop growth model and decision support system (DSS).
  • To initiate model development for grape.
  • To develop decision support system for improving crop productivity under moisture and temperature stress conditions.

01.06.2012 to 30.06.2016

Development of Decision support system will improve the farmer’s ability to take crucial management decisions keeping the economics and long term prospects of the standing crop.  Moisture and high temperatures stress –agro informatics database of the study area

Studies on Crop Model simulating grape growth and yield will be initiated to develop crop model

Web and mobile based Decision Support System for enhancing crop productivity under moisture and high temperature stress conditions will be developed.

194.84

Techniques to Improve Nutrient Use Efficiency Including Farm Waste.

J. Sharma

A.K.Sharma

Indu Sawant

·       To standardize nutrient doses for important varieties of grapes.

·       To evaluate different sources of fertilizers for their suitability for grapes.

01.04.2007 to 30.06.2013

Different sources of fertilizer including pruned biomass, methods of fertilizer application including foliar sprays will be tested for improving nutrient use efficiency. Nutrient doses for table and wine grapes will be standardized.

49.59

Developing petiole nutrient guides for Cabernet Sauvignon grapes

J. Sharma, PI

A.K. Upadhyay

A.K. Sharma  

·       To develop petiole nutrient guides for Cabernet Sauvignon vines raised on Dogridge rootstock

01.04.2008 to 30.06.2014

Petiole nutrient guides for Cabernet Sauvignon raised on 110R rootstock vines will be developed for bud differentiation (foundation pruning) and (fruit pruning) stages. Diagnosis and management of new nutritional disorders will be done.

61.91

Standardization of growth regulator schedule for new grape varieties

S.D. Ramteke, PI

Kaushik Banerjee 

  • To standardized the dose of plant growth regulator for new promising varieties (Red Globe, Crimson Seedless, Fantasy Seedless, Manjri Naveen, Autumn Royal and Autumn Seedless)
  • To determine the residue levels of PGR’s at different stages in selected new varieties

01.10.2012 to 30.09.2015

 

63.00

Physiological disorders and their management practices in grapes

S.D. Ramteke, PI

A.K. Upadhyay

S.D. Sawant

R.G. Somkuwar

Kaushik Banerjee 

  01.10.2011 to 30.09.2017   44.50
Isolation, bio-efficacy evaluation, characterization and formulation of viticulturally important micro-organisms Indu S. Sawant, PI   01.01.2006 to 30.06.2016    
ORP on Diagnosis and Management of Leaf-spot Diseases of Horticultural and Field Crops – Grapes Indu S. Sawant, PI   19.06.2009 to 31.03.2013    

Studies on the biology and control of the fungi causing anthracnose disease in grapes

Indu S. Sawant, PI

S.D. Sawant

G.S. Karibasappa
Kavita Y. Mundankar 

·       To confirm the pathogen(s) of the anthracnose disease.

·       To study the morphological, molecular and pathological diversity in the pathogen.

·       To study the Host-Pathogen-Environment interaction in field.

·       To identify potential bio-control agents & new generation fungicides.

·       To develop technologies for producing disease free planting material.

·       To identify anthracnose resistant / tolerant germplasm.

·       To identify anthracnose resistant / tolerant germplasm.

01.06.2009 to 31.12.2014

The project aims at generating information on the anthracnose pathogen(s), the diversity within the organism, its epidemiology, survival and sensitivity to new generation fungicides and common bio-control agents. It will also identify the sources of resistance in grape germplasm at NRCG. The project will result in ways to manage the disease more effectively in vineyards and nursery; technology to production anthracnose free planting material; and to identify sources of resistance in germplasm.

110.35

ORP on “a holistic analysis for the absence of Botrytis cinerea infections in grapes.

Indu S. Sawant, PI

S.D. Sawant

 

·       To confirm the absence of Botrytis cinerea infections in grape.

·       To collect and analyse available weather data at flowering and after varaision and correlate to epidemiological requirements.

·       To check for presence of spores of Botrytis cinerea in vineyards.

·       To check for presence for Botrytis cinerea in imported grapes.

·       To isolate Botrytis cinerea from other hosts in Maharashtra.

·       To check the pathogenicity and virulence of different isolates of Botrytis cinerea on grapes.

·       To study variability within isolates from different hosts.

·       To analyse, report and document the data.

01.01.2012 to 30.06.2014

The project aims at confirming the absence of Botrytis cinerea in grapes in Maharashtra and finding reasons for its absence based on analysis of available information.

10.00

Studies on virus diseases of grapes

S.D. Sawant, PI

Indu S. Sawant

Amala U.

J. Sharma 

·       To conduct survey for presence of virus diseases.

·       To study the distribution and everity of different strains of GVLRaV in wine and table grape.

·       To standardize more economical methods for detection of different strains of GVLRaV.

·       To study the effect of GVLRaV 1+3 on yield and quality of table grapes and wine in case of wine varieties.

·       To study the transmission of GVLRaV.

·       To develop management strategies for virus diseases

01.04.2007 to 31.03.2014

 

 83.50

Studies on bio-efficacy of fungicides and safer environmental profiles products for management of grape diseases

S. D. Sawant, PI
Indu
S. Sawant

Kaushik Banerjee

·       To study the bio-efficacy of new fungicides and products with safe environmental profile in control of common diseases in grapes

·       To study the terminal residue of promising fungicides and products with safe environmental profile

·       To study the effect of promising fungicides and products with safe environmental profile, on useful micro-organisms and insects

·       To optimize the use of appropriate fungicides in vineyards

·       To Study iatrogenic effects of pesticides

01.04.2007 to 31.03.2013

 

28.35

Development and testing of disease forecasting models and development of pest alert systems

S.D. Sawant, PI

Indu S. Sawant

Kavita Y. Mundankar
J Sharma

·       To Develop weather data based disease forecasting model for downy mildew, and anthracnose which can be used using weather data recorded on any weather station.

·       To develop expert system to generate location specific disease management advisory for downy mildew, powdery mildew and anthracnose in grapes.

01.04.2007 to 31.03.2014

 

27.75

Studies on the management of mites

 

D.S. Yadav, PI
Mrs Amala U.
Indu S. Sawant

·       To survey the grape growing areas to know the seasonal incidence, identify new species and their natural enemies.

·       To study the influence of weather parameters on mites and to develop prediction modules.

·       Evaluation of promising non-chemical methods of insect pest management.

·       Evaluation and utilization of bio-pesticides.

·       Evaluation and utilization of different neem formulations and other botanicals.

·       Evaluation of new generation molecules for mite management.

·       Compatibility of bio-control agents and selected pesticides.

·       To develop an integrated pest management (IPM) strategy.

01.04.2008 to 31.03.2013

This project is aimed at studies on the management of mites in grapes. This project is also aimed at surveying mites and their seasonal incidence and establishing its relationship with weather parameters and to develop prediction modules. Field evaluation of the bioagents, botanicals and the insecticides is being carried out. The laboratory studies are being conducted to evaluate the compatibility of the pesticides to the bioagents. The ultimate aim of the project is to bring out an integrated pest management package for mites in grapes.

16.62

Studies on the management of thrips

D.S. Yadav, PI

Amala U.

S.D. Sawant 

·       To survey the grape growing areas to know the seasonal incidence, identify new species and their natural enemies.

·       To study the influence of weather parameters on thrips and to develop prediction modules.

·       Evaluation of promising non-chemical methods of insect pest management

·       Evaluation and utilization of bio-pesticides

·       Evaluation and utilization of different neem   formulations and other botanicals.

·       Evaluation of new generation molecules for thrips management

·       Compatibility of bio-control agents and selected pesticides.

·       To develop an integrated pest management (IPM) strategy.

01.04.2008 to 31.03.2013

This project is aimed at studies on the management of thrips in grapes. This project is also aimed at surveying thrips and their seasonal incidence and establishing its relationship with weather parameters and to develop prediction modules. Field evaluation of the bioagents, botanicals and the insecticides is being carried out. The laboratory studies are being conducted to evaluate the compatibility of the pesticides to the bioagents. The ultimate aim of the project is to bring out an integrated pest management package for thrips in grapes.

20.42

Insect biodiversity in grapevine ecosystem with emphasis on economically important grape pests

Amala U, PI

D.S. Yadav

·       To collect and identify the different insect species associated with grapevine ecosystem.

·       To preserve and display of various grape insects for future study and creating awareness about grape insects among the grape growers

·       To gather various information’s which are relevant for the management of economically important insect pests of grapes.

·       To identify the naturally present beneficial insect fauna for use in IPM.

·       To monitor the entry of invasive pests

01.12.2010 to 30.11.2013

Project aims at the collection of all the insect fauna associated with the grapevine ecosystem and their identification. GPS information and season of collection (including plant growth stage and cultivar) would also be collected for future reference. It also aims at their preservation and making repository for display of different stages for posterity. The study will help in the generation of information like biology of major pests, injurious stage of the pest to the crop, their nature of damage, different parasitoids and predators, available options for their management and their distribution within India. This also helps to maintain a good data base, which of course is the backbone of any scientific research. Ultimately this project helps in effective and bio intensive management of major grape insects by exploring the various natural enemies available to manage them and thereby reducing the pesticide load on the crop.

33.62

Multi-pronged strategy for the management of mealybugs in grapes.

D.S. Yadav, PI
Mrs Amala U.

Kaushik Banerjee

·       To study the chemical composition of the waxy covering of Maconellicoccus hirsutus.

·       To explore the surfactants effective in removing mealy covering to enhance effectiveness of the insecticides.

·       To explore potential of hydro-dynamics for the management of mealybugs.

·       To study mealybug population trends and seasonal development of different mealybug species.

·       To develop mealybug population risk assessment model.

·       To identify alternate host plants in the vineyards.

·       To evaluate new generation chemicals for bio-efficacy against mealybugs, phytotoxicity and compatibility with other chemicals.

·       To evaluate various potential biological control agents for mealybugs and their compatibility with pesticides.

01.04.2012 to 31.03.2017

·      Identification of waxy covering and effective surfactants to remove this defence mechanism will enhance the effectiveness of contact insecticides.

·      Study on hydro-dynamics will simulate impact of rain droplets and will be helpful in providing a novel mechanical method to control mealybugs.

·       Studies on seasonal incidence will be used to develop mealybug risk assessment model which will also incorporate automated weather forecasting based advisory to farmers.

·      Identification of alternate host plants of mealybug in viticulture ecosystem will be helpful in reducing breeding areas of mealybugs.

·      Identification of new generation chemicals for incorporation in package of practices

 36.00

Persistence studies of agrochemical residues in soil and water

Kaushik Banerjee, PI

Ahmmed Shabeer T.P.

·       Dissipation studies in soil and water to estimate the rate of degradation

·       To investigate the adsorption-desorption of agrochemicals on soil Exploring fate of pesticides while processing of grapes to raisin and wine

·       Management of agrochemical residues in soil and water bodies to minimize agrochemical residues in grapes

01.04.2008 to 31.03.2014

Persistence and dissipation of the residues of new generation pesticides in soil of different physico-chemical properties and water with different pH and EC will be explored under in vitro and field conditions. Residues will be monitored on a regular basis at different time intervals after treatment. Dissipation rate kinetics will be studied to estimate half-life on the basis of linear and non-linear kinetic models. Adsorption-desorption will also be simultaneously studied to evaluate the risk of groundwater contamination through leaching.

28.93

Studies on dissipation rate of new generation pesticides with reference to changing MRL

Kaushik Banerjee, PI

Ahmmed Shabeer T.P.

·       Monitoring of pesticide residues in grapes at different time intervals from the time of final application till harvest

·       To prescribe safe waiting period for different pesticides in grapes

01.04.2008 to 31.03.2014

Persistence and dissipation of the residues of new generation pesticides will be explored. Residues will be monitored in grapes on a regular basis at different time intervals after final spraying till harvest. Dissipation rate kinetics will be studied to estimate half-life and pre-harvest interval (PHI) of pesticides in grapes on the basis of latest and minimum MRL available at international level. In case of older pesticides, PHI will be re-determined by conducting dissipation studies with regards to new MRL.

28.93

Monitoring of agrochemical residues in grape and grape produce

Kaushik Banerjee, PI

Ahmmed Shabeer T.P.

·       Monitoring of pesticide residues in grapes and raisins from vineyards, pack houses, cold storages and local markets.

·       Monitoring of pesticide residues in wine.

·       Exploring fate of pesticides while processing of grapes to raisin and wine

01.04.2008 to 31.03.2014

Agrochemical residues in grapes and grape processed products viz. raisin, juice and wine from different sources viz. vineyards, export pack houses, wineries, domestic markets, etc. will be explored. Fate of pesticides during processing of grapes to raisin and wine will be investigated. The estimated residues will be compared with the ADI and MRL values for evaluation of food safety to the consumers. The results will work as the foundation for IPM schedule/recommendations for grapes to ensure safe use of pesticides.

28.93

Comprehensive screening of Target, non-target and unknown emerging organic contaminants in fruits and vegetables by GC-MS and LC-MS

Kaushik Banerjee, PI

  • Develop and validate multiresidue methods
  • To determine occurrence of emerging organic contaminants
  • Characterization of primary and secondary metabolites
  • Fate of pesticides and mycotoxins in fruits and vegetables
  • Safety evaluation of food materials
  • Developing mitigation strategies according to mapping of important and vegetable growing region

01.04.2011 - 31.03.2015

Development and validation of non-targeted screening methods for analysis of pesticides, mycotoxins and emerging contaminants in fruits and vegetables

Monitoring of fruits and vegetables grown in India

49.90

Profiling  of grape varieties Shiraz and Sauvignon Blance for its phenolic and aroma compounds from grape to wine

Ahammed Shabeer T.P. , PI

Kaushik Banerjee,

J. Satisha

A.K. Sharma

·       Standardisation and validation of methodology for the extraction and analysis of phenolic and aroma compounds from grapes and wine.

·       Phenolic and aroma compound profiling of wine grapes Shiraz and Sauvignon Blance varieties collected from different locations after the harvest.

·       Phenolic and aromatic profiling of wine and waste generated from Shiraz and Sauvignon Blance during wine making and aging process.

01.07.2012 to 31.07.2014

A method for the extraction and analysis of phenolic and aroma compounds in LC-MS/MS and GC-MS, respectively will be standardised and validated (work just started). The grape varieties Shiraz and Sauvignon Blanc will be collected from different locations and the profiling for phenolic and aroma constituents will be done for different parts (skin, seed and pulp). The same collected samples will be used for wine making and the phenolic and aroma profiling of the wine, changes during the aging and the waste generated during the process also will be evaluated. Further, a processing factor from grapes to wine will be generated for each phenolic and aroma compounds

36.90

Standardization of pre-harvest factors for production of quality red wines

A.K. Sharma, PI

R.G. Somkuwar
Kaushik Banerjee

·       To standardize the pruning time.

·       To standardize the crop load.

·       To identify the proper maturity.

01.01.2008 to 31.12.2013

Twenty years before there was no Indian made wine was available with drinkable quality. All hot countries like India, Brazil, South Africa, Thailand etc. are relatively new to wine making and wines produced from tropical regions are known as new world wines. In the tropical regions like India, both local and old world wines are clearly extremely vulnerable to poor storage and transport conditions. The main constraint in achieving the quality goal in our country is fact that the quality compliances are very poor among the wine manufactures. In order to understand the export potential and improvement in domestic consumption of Indian wines it is of utmost importance to address quality related issues. The wine technology in India is transferred from temperate regions. The technical involvement for production of wine in India is adopted as such which developed in temperate regions. However, our grape production falls in tropical climatic conditions.  So we can’t follow the maturity indices as well as harvesting time, which is developed from temperate conditions. The harvesting of berry at proper maturity and harvesting time have prime role in the production of quality wine. The quality parameters of grape berries at harvesting time directly affect the quality attributes of wine such as alcohol percentage, acidity, aroma compounds etc.

42.23

Standardization of techniques for minimization of browning in raisins

A.K. Sharma, PI

S.D. Ramteke

Kaushik Banerjee

·       To standardize the concentration of potassium carbonate and ethyl oleate.

·       Standardization of treatment duration.

·       To study the effect of anti PPO substances on quality of raisins.

01.04.2008 to 31.03.2013

Grapes are one of the most popular and palatable fruits in the world. The preservation of grapes by drying is a major industry in many parts of the world where grapes are grown. Drying practices vary with geographical locality and with the variety of grapes. Drying grapes, either by open sun drying, shade drying or mechanical drying, produces raisins. Air-drying of solar energy has been demonstrated to be cost-effective and could be an effective alternative to traditional and mechanical drying systems, especially in locations with good sunshine during the harvest season. The raisin colour is deciding factor for the price of processed produce. In world market, green coloured raisins catch more prices.  In India, mainly yellow or brown coloured raisins are being produced. Enzymatic and non-enzymatic activities are responsible for browning of raisins. High humidity at the time of grape dying (due to rains), content of polyphenols in berries, prevailing temperature, exposure to direct sunlight etc. are responsible factors for raisin browning. In the last grape drying season, Solapur and Sangli regions faced untimely heavy rains resulted in heavy loss of produce due to browning. During grape drying process, sulfur fumigation leads production of yellow coloured raisins.

26.10

Development of databank on grape

Kavita Y. Mundankar, PI

·       To compile data related to production, trade, and inputs required for grape production and processing, and putting it in a form for ready access

01.01.2007 to continuing project

 Data has been collected from different sources on grape cultivation, harvest and utilization, packing and storage, processing techniques, government policies, area of cultivation, production and trade, quality standards etc.

Data on area and production was collected from National horticultural board (NHB) and State departments and trade data was collected from Agricultural and Processed Food Products Export Development Authority (APEDA) website. 

A format for data collection was devised and correspondence was done with grape growers’ associations and state departments of various states and NHB for collection of data.

Data has been collected on district wise area and production, variety wise area and production, and market information on whole sale price of fresh grapes in different markets of the state from state horticulture departments.

Area, production and trade data of major grape growing countries was collected from the Food and agriculture organization (FAO) website.

Relevant data/information was compiled for display on website

A database on varieties/accessions distributed by the Centre under material transfer agreement was created along with the facility to store and retrieve the data reports.

 

NRCG - DIPS -  A system for diagnosis and management of important diseases and insect pests of grapes

Kavita Y. Mundankar, PI

Indu S. Sawant

S.D. Sawant
D.S. Yadav

Amala U. 

·       To develop a computerized database of grapevine diseases and insect pests under Indian agro-climatic conditions

·       To develop a user-friendly diagnosis and management system for grapevine diseases and insect pests under Indian agro-climatic conditions.

01.05.2008 to 31.12.2010

Proposed for extension upto September 2013

System analysis was done and user requirements were identified, a logical model of the system was developed and information on grapevine insect pest has been compiled and coded using HTML for display.

Information on grapevine diseases was compiled and graphical user interface was designed for the e-book on grapevine diseases. The web-pages were coded using HTML/DHTML/Javascript script for display in the browser. Analysis and designing of the grapevine disease and pest problem diagnosis was initiated. 

12.63