Proteomic analysis of Thompson Seedless grapes grafted on different rootstocks at different phenological stages of growth and development
Name of PI with associates
Dr. J. Satisha, Sr. Scientist (Horticulture)
Dr. Anuradha Upadhyay, Sr. Scientist (Biotechnology)
Dr. K. Banerjee, National Fellow (ICAR)
Objectives:
To characterize the proteomic profile in Thompson Seedless grapes at different stages of vine growth and development.
To study the effect of rootstocks in influencing the protein profile in Thompson Seedless at different phenological stages.
- To understand the overall mechanism of rootstocks in controlling vegetative, reproductive and quality attributes of scion varieties with respect to their role played in regulating different proteins / enzymes.
Duration:
29 April 2011 to 26 December 2014
Highlights:
Proteome is the entire set of proteins expressed by a genome, cell, tissue or organ. It is the set of expressed proteins in a given type of cell or an organism at a given time under defined conditions.
Grapevine is a woody vine that produces the most economically important fruit crop in the world. They contain wide range of proteins, which assumes a considerable technological and economic importance because they greatly affect the quality of grapes and wines. Most important are chitinase, thaumatin like proteins and osmotins (Monteiro et al., 2001). The protein present in the grapes is dictated by several factors such as cultivars, cultural practices, soil, climate, period of the year and rootstocks (Baily and Berg, 1967 and Moretti and Berg., 1965).
Grapevine proteomic profiles have been characterized by proteomic studies (Tesnierress and Robin, 1992, Vincent et al., 2006). Though many methodologies have been developed for characterization of protein profiles, two dimensional electrophoresis (2 DE) remain one of the most efficient strategies to isolate proteins showing qualitative variation in response to a treatment (Gorg et al., 2004).
In India commercial table and wine grape cultivars are being grafted on drought and salt tolerant rootstocks to overcome the adverse effects of soil salinity and water scarcity. Our previous studies on influence of rootstocks on performance of commercial table grape cultivars has shown that rootstocks apart from overcoming these adverse effects of abiotic stresses also influenced several morphological, physiological and biochemical aspects. Same cultivars when grafted on different rootstocks have performed differently with respect to time of bud burst, fruit bud differentiation, yield per vine, on set of veraison, days to maturity and fruit ripening etc. Hence, this study projects to characterize the protein profile of Thompson Seedless grapes grafted on different rootstocks at different phenological stages of vine growth and fruit development using proteomic approach. .
Total outlay: Rs. 66.51 lakhs