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Management of  Grape Genetic Resources  of  table, wine, raisin, juice and rootstock varieties - Phase II

 

Name of PI with associates

Dr. G.S. Karibasappa, Pr. Scientist (Horticulture)

Dr. Anuradha Upadhyay, Sr. Scientist (Biotechnology)

Dr.A.K.Sharma, Sr. Scientist (PHT)

Dr.A.K.Upadhyay, Sr. Scientist (Soil Science)

Dr. Indu S. Sawant, Principal Scientist (Plant Pathology)

Dr. S.D. Ramteke, Sr. Scientist (Plant Physiology)

Dr. D.S. Yadav, Scientist (Entomology)

Objectives:

• To evaluate cultivars as active collections and to conserve desirable genotypes on long term basis through cryo-preservation.

• To exchange and coordinate the grape improvement programme as a nodal centre in the country.

• To develop improved cultivars/hybrids through non-conventional hybridization programmes such as somatic hybridization, embryo rescue and genetic engineering for improved berry and bunch characters, higher tolerance (immune) to dreaded diseases (mildews) and saline water.

Duration:

April 2008 to May 2013 (Phase II)

Highlights:

This project forms a basis for establishing a sound national germplasm repository for grapes at NRCG, Pune. Collection of clonal material will be expedited through surveys and explorations and also augmentation from the previous collections from different AICRP(STF) grape centers in the country. Thrust will be given for tolerant types to various biotic and abiotic stress. The maintenance of clonal material will be in the form of active field   gene bank on midterm basis (12-15 years). However, long term  germplasm  conservation  efforts will be taken up, when suitable facilities and expertise  for tissue culture /cryopreservation will be established.

The key objectives of the project are to ;(similar as in phase I, but applicable for  new & remaining  accessions  made during phase II)

 1.   establish gene bank of various traits of economic importance,

2.   identify suitable donors for biotic and abiotic stress tolerance,

3.   establish relevant molecular markers for cultivar identity and diagnostics,

4.   evolve improved cultivars from the base (check) Thompson seedless or introduce superior genotypes widely acceptable for commercial cultivation.

 All the accessions will be maintained  as grafted plants on a standard rootstock (DogRidge).These  will be identified in separate groups such as ;

a). Table grapes

b)  Raisin grapes

c). Wine grapes

d). Juice grapes

e) rootstocks

f)  Closely related  species/genera  for  use  in grape improvement  through hybridizations and gene transfer.

The planting will be made with 6 vines  per accessions, spaced 10’ x 6’  and trained  on  ‘Y’ trellis system. Standard UPOV descriptors for grapevine, published by IBPGR, Rome will be followed for  Passport information, characterization and evaluation studies and for documentation of the genotypes.  Molecular markers like RFLP’s, AFLP’s, ISSR’s and SSRP’s will be used to characterize the germplasm. The genotypes will be scored/enumerated   using  weighted score card for different traits before identifying them as promising and  recommendations acceptable for commercial cultivation. The results will be obtained for at least 3 harvest seasons before making  conclusions. The second  phase of the project will be reported after 5 years of its commencement, although the very nature of the project is infinite and in program mode. The entire funding of this project will be  made 100 per cent  by the Indian Council of Agric. Research, New Delhi through plan and non-plan schemes of NRCG, Pune. However attempts will be made to obtain adhoc projects and sponsored schemes of  ICAR/DBT/ APEDA.

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Total outlay:

A. Recurring : 1.  Salaries :                                                                                        (Rs.lakh) S. No Incumbent 1st year 2nd year 3rd year 4th year 5th year+ Total i) Scientific 9.60 10.50 11.50 12.25 13.00 56.85 ii) Technical 3.00 3.20 3.50 3.75 4.00 17.45 iii) Supporting 2.00 2.10 2.50 2.75 2.90 12.25 iv) Wages 0.80 0.90 1.20 1.50 1.80 6.20   Sub Total 15.40 16.70 18.70 20.25 21.70 92.75 2. Consumables S. No Particular 1st year 2nd year 3rd year 4th year 5th year+ Total i) Lab Chemicals 0.15 0.50   1.00 1.25 1.00   3.90 ii) Farm  inputs 2.00 2.20 2.50 2.75 2.00 11.45 iii) Glass/plastic  wares 2.00 2.00 2.00 2.00 2.00 10.00 iv) Others 0.80 0.90 1.00 1.50 1.50   5.70   Sub Total 4.95 5.60 6.50 7.50 6.50 31.05   3. Travel & HRD: S. No Particular 1st year 2nd year 3rd year 4th year 5th year+ Total i) Travel 0.25 0.75 1.25 2.00 1.50 5.75 ii) HRD training 0.75 1.50 2.50 2.50 2.00 9.25   Sub Total 1.00 2.25 3.75 4.50 3.50 15.00 4. Miscellaneous    0.50 0.50 0.50 0.50 0.50   2.50 Sub total A (Recurring) 21.85 25.05 29.45 32.75 32.20 141.30 B.  Non-Recurring (Equipments & plant material)

Particular 1st year 2nd year 3rd year 4th year 5th year+ Total i) Portable mist chamber 7.50 ---- ---- ---- ---- 7.50 ii) Importation of planting material 5.00 5.00       10.00   Sub Total B 12.50 5.00       17.50

Project  Total (A and B)                                               Rs. 158.80  Lakhs